FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34129122
Sequences flagged as poor quality0
Sequence length126
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA279550.0111.0164261
AGGCGAC143850.0110.817298
TGACTAG206350.0109.8537141
CGGACGT197350.0109.5111161
ATCGACG132250.0108.470039
AGAGCGA158200.0108.348761
ATAGACC148450.0107.864321
ATAACGC189300.0107.6686551
CGCCGAC132100.0107.4425662
GCCGACA136800.0107.423463
ACAGCCG146900.0107.327361
GGCGACA148700.0107.243229
CAATGGA223350.0107.15298
ATTCTAC381250.0106.873031
ACTGAAC186750.0106.7285161
CGACGGA95850.0106.222279
GCAGCGA115150.0106.02946
GAGGCGA87550.0105.876167
CCTTCGA261450.0105.70931
TGAGCCG121700.0105.534731
Produced by FastQC (version 0.11.5)