FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34129122
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG348550.10212685811255268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1577150.091.649671
AGCAGTG1670400.086.435812
GGTATCA1821400.079.0870368
GTGGTAT1832150.078.777386
TGGTATC1851550.077.857657
GTATCAA1867400.077.315589
CAGTGGT1925300.075.134244
AGTGGTA2047400.070.47185
GCAGTGG2262500.064.1935653
CAACGCA1757000.044.8534812-13
ATCAACG1776450.044.4050310-11
AGAGTAC1850100.041.93512718-19
TCAACGC1749750.041.30362312-13
TACATGG1854750.040.90020822-23
AGTACAT1909300.040.36480720-21
CGCAGAG1818150.039.68891516-17
GCAGAGT1984000.039.3726216-17
GAGTACA1829400.038.77754220-21
TATCAAC1871900.038.66803710-11
GTACATG1844250.038.1416622-23
Produced by FastQC (version 0.11.5)