FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31549678
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1645950.097.08281
AGCAGTG1790250.089.1249852
GTGGTAT2077000.076.7181246
GGTATCA2079750.076.6107948
CAGTGGT2127050.075.006024
TGGTATC2127100.074.888497
GTATCAA2142950.074.463389
AGTGGTA2148750.074.131275
GCAGTGG2340000.068.31863
CAACGCA2018150.043.47281612-13
ATCAACG2034300.043.2004910-11
TACATGG2089650.040.47439222-23
AGTACAT2148850.039.89958220-21
AGAGTAC2163250.039.898718-19
ACGCAGA2207950.039.79286214-15
TCAACGC2007100.039.751512-13
GCAGAGT2185600.039.6883816-17
CGCAGAG2079550.038.29919416-17
GAGTACA2086150.037.56986220-21
GTACATG2092900.037.14771322-23
Produced by FastQC (version 0.11.5)