FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33412969
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG640130.19158129886631745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC499250.14941802986738473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2555500.0104.445051
AGCAGTG2810100.094.900892
CAGTGGT3375200.079.023394
GTGGTAT3401100.078.3317646
GGTATCA3430600.077.582868
TGGTATC3434750.077.471667
GTATCAA3472700.076.745989
AGTGGTA3493050.076.2079545
GCAGTGG3686550.072.544833
CAACGCA3364300.043.87689212-13
ATCAACG3378700.043.7517410-11
AGAGTAC3454050.042.1332718-19
TACATGG3454300.041.44154422-23
AGTACAT3506200.041.2777320-21
GCAGAGT3550600.041.16933416-17
ACGCAGA3641250.040.56684514-15
TCAACGC3364500.039.67391612-13
CGCAGAG3433850.038.7530916-17
TATCAAC3464500.038.5500610-11
GAGTACA3436200.038.2039320-21
Produced by FastQC (version 0.11.5)