FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34070790
Sequences flagged as poor quality0
Sequence length126
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA196650.0110.511971
CGGACGT205250.0109.741311
ATGCGAG93400.0109.028233
TGGACGC145800.0108.055411
GACTACA209050.0107.114881
ACGGAGA153850.0107.0437551
TGACGGC123150.0106.632021
AGCGTCG102450.0106.443151
CATGCGA96000.0106.023142
ACAGCGA114950.0105.7973566
CAGCCGA107450.0105.455761
GCGAGAA134150.0104.8229451
TCCGTAG159650.0104.5110248
CCTTCGA181900.0104.427711
AGCACCG156600.0104.056228
GGACGCA86700.0103.4827962
GCTGCGA95900.0101.822481
CTCGAGG118600.0101.7152561
AGATGTG156150.0101.589668
GATGCTA118800.0101.544021
Produced by FastQC (version 0.11.5)