FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34070790
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG503750.14785392413853626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC367420.10784017629177368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2299300.0102.320831
AGCAGTG2493050.094.2141652
CAGTGGT2993950.078.427664
GTGGTAT3066450.076.5186166
GGTATCA3069700.076.3436668
TGGTATC3101550.075.480387
GTATCAA3125650.075.084599
AGTGGTA3127700.075.000965
GCAGTGG3207800.073.3769153
CAACGCA3009100.042.5688812-13
ATCAACG3021550.042.4773710-11
AGAGTAC3098050.040.52721818-19
TACATGG3063100.040.24958822-23
AGTACAT3137400.039.79824420-21
GCAGAGT3176950.039.75773216-17
ACGCAGA3268450.039.19756714-15
TCAACGC2999050.039.15365212-13
CGCAGAG3069450.038.11549416-17
GAGTACA3048750.037.6856920-21
TATCAAC3158700.037.25207510-11
Produced by FastQC (version 0.11.5)