FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33124236
Sequences flagged as poor quality0
Sequence length126
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGC141850.0109.184481
TACGGAT245450.0108.785831
GAACCTA227350.0108.4426961
GTCCGAA165850.0108.343148
GCGCGAT163750.0107.339681
AGTGAAC163950.0107.2933048
ACGGATG143250.0106.970652
GAGGACG121400.0106.499769
CGACACA189350.0105.6700445
AATCGAC125150.0104.555326
ATCGACC125250.0104.232037
CTAGTGA115100.0103.736121
TGACTAG120350.0103.700041
AGGCTAG169250.0103.614428
ACGATAC139800.0102.927491
TGACGGC92650.0102.825791
GACTAAC121600.0102.584691
AACCGCG184750.0102.5145341
AGCTTGA173050.0102.4731
TGCATCG147750.0102.368586
Produced by FastQC (version 0.11.5)