FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30286652
Sequences flagged as poor quality0
Sequence length126
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGC125300.0109.182581
TGACTAG152400.0108.3591841
CGCTTCA177200.0105.8974151
GTCCGAA116850.0103.715398
ACGAGCC95150.0103.276141
TTCGCGA98250.0103.137759
AGTGATA118100.0103.125657
ACGAGGA114650.0102.308241
ATGCGAT121150.0102.220021
ACACTGA166600.0101.5374151
CCTTCGA158700.0101.296331
CGGACTG74450.0100.948471
TACACGA130300.0100.892711
CTAAGAC88650.0100.555961
CACGGTA103550.0100.118468
CGGAGCT96000.099.610391
ACTGATG91850.099.60151
TGGACGC75150.099.528891
CTCGAAA112800.099.249851
AGTGAAG113500.099.111948
Produced by FastQC (version 0.11.5)