FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30286652
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1296400.090.915271
AGCAGTG1430250.082.0005652
CAGTGGT1795050.065.0345464
AGTGGTA1874900.061.9300845
GTGGTAT1904850.061.0103656
GGTATCA1939150.059.7642178
GCAGTGG1973200.059.4999923
TGGTATC1958300.059.250257
GTATCAA2013000.057.7862789
CAACGCA1881350.034.5856712-13
ATCAACG1922300.033.93330810-11
AGAGTAC1952550.032.24135618-19
TACATGG1906050.031.88543322-23
AGTACAT1971650.031.42635220-21
GCAGAGT2031850.031.15792516-17
ACGCAGA2107350.030.82939114-15
TCAACGC1887550.030.71442612-13
CGCAGAG1945100.029.57647716-17
GAGTACA1911650.029.21256420-21
TATCAAC2018850.028.75889210-11
Produced by FastQC (version 0.11.5)