FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34192924
Sequences flagged as poor quality0
Sequence length126
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC115550.0106.608051
ATGCGAT142250.0104.997921
CGTTAGA156500.0104.835371
AGACTAG112000.0104.404788
CGAACAT217950.0103.675311
AGAGCGA111400.0103.5740051
TGCGCAG148150.0102.8019561
TGACGGC91300.0102.771041
CGCTGGA108350.0101.392241
CGTGAGC97600.0101.180921
CAATGGA157050.0101.0830848
AGATGCA123000.0101.017763
GCATGCG104600.0100.895031
ACGCCGA99000.0100.295841
GGCGACT82050.099.504541
CTCTACG148850.099.092071
GATGCGA56550.098.668139
CTCGGAG146600.098.5654451
CTTAGGA129050.098.5259251
GCAGCTA83050.098.393256
Produced by FastQC (version 0.11.5)