FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37105726
Sequences flagged as poor quality0
Sequence length126
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG301000.0113.079931
ATAGACC209050.0110.899881
CTGCGGA200300.0110.859361
CTGATAG177950.0107.814651
TGACGGC124650.0106.720151
TGACTAG179500.0106.21481
GCGACCA262600.0105.9784851
TCGCGAG124450.0105.251631
GACGTGC191600.0105.209311
CGGGATA139100.0104.9880752
CATGGCG160200.0104.884031
CGATGGC260850.0104.756361
GACCAGT207650.0104.2140353
TGGACGC138500.0103.8932341
GTATAGG203700.0103.5855568
CCGATAG143300.0103.2844543
GTACGTA181100.0103.121821
ACACTGA167950.0102.653421
TGCTACC203100.0102.5329361
AGCGTCG70650.0102.471751
Produced by FastQC (version 0.11.5)