FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37105726
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1361000.087.0518651
AGCAGTG1497900.078.558762
CAGTGGT1822750.064.165934
GTGGTAT1876550.062.1159676
GGTATCA1897700.061.2276428
TGGTATC1939450.060.070497
AGTGGTA1968350.059.2454075
GTATCAA1971700.059.1548969
GCAGTGG2060700.057.079733
CAACGCA1820200.035.8472112-13
ATCAACG1847350.035.42044410-11
AGAGTAC1928700.032.69293218-19
TACATGG1863300.032.68634822-23
TCAACGC1807550.032.18235412-13
GCAGAGT1985550.031.95742416-17
AGTACAT1964050.031.65327320-21
CGCAGAG1890000.030.68837416-17
ACGCAGA2154650.030.36612114-15
GAGTACA1861250.030.11151720-21
TATCAAC1973000.029.59901410-11
Produced by FastQC (version 0.11.5)