FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31832718
Sequences flagged as poor quality0
Sequence length126
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGT150150.0108.2339251
CCGTGGA140900.0108.053491
TAAGCTA164300.0106.152676
TAGTACG160800.0105.776827
CTGCGGA105750.0105.706041
CCAGCGA120400.0105.459181
CGAGGAC90900.0105.3388062
TTACGGA141450.0104.425999
AACACGC159050.0103.960659
GACAGCG98250.0103.4437566
ATGCGAT125750.0102.929831
GACCGGA77950.0102.5207069
TGCGCAG170000.0101.4229051
GATGCTA105650.0101.317011
TCGTAGA98750.0101.222641
TGACGGC68700.0101.1934361
CGTGAAG72550.0101.054877
CGAACAT174600.0101.054541
TGACAAC141150.0101.014391
GCGTGGA75300.0100.948762
Produced by FastQC (version 0.11.5)