FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31832718
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG542250.17034360685129055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC435210.13671782598017548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1288950.090.166861
AGCAGTG1405650.082.3879552
CAGTGGT1670900.069.188664
GTGGTAT1714150.067.060316
GGTATCA1734900.066.4069758
TGGTATC1750150.065.63297
GTATCAA1784050.064.496769
GCAGTGG1955300.059.4794353
AGTGGTA2138750.053.964755
CAACGCA1658550.038.44414512-13
ATCAACG1677600.038.0021810-11
AGAGTAC1749600.035.54855718-19
TACATGG1723300.034.87912822-23
AGTACAT1772650.034.74461420-21
TCAACGC1654850.034.64319212-13
GCAGAGT1861550.033.67880616-17
CGCAGAG1714400.033.60503816-17
GAGTACA1709900.032.7614320-21
GTACATG1713450.032.25015622-23
TATCAAC1809800.031.83923310-11
Produced by FastQC (version 0.11.5)