FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32846435
Sequences flagged as poor quality0
Sequence length126
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAAATTGTTTC416630.12684177141294026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG156300.0108.388391
TGACGGC92100.0104.877221
CGAGCAC113700.0104.858471
ACGAGGA120050.0104.662671
CTGCGGA104100.0103.917521
TCCACGA163050.0102.624088
AGCTACC114450.0102.168638
ATCGTGG115700.0101.635227
CCTTACG159850.0101.399511
CATCGAA133300.0100.699291
CGAACAT189050.0100.313111
GACTGCG120200.0100.1370241
CGTAACA112250.0100.001773
GACTAAC92750.099.935121
GAACCTA147650.099.166071
CGGCTAG134250.098.779351
GCCTCTA188400.098.7473451
AGGTCCG157900.098.393611
ACGAGCC83000.098.293791
CTGATCC158500.098.248391
Produced by FastQC (version 0.11.5)