FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32846435
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG688620.20964832256529514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC542750.16523863244215087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1369300.091.651661
AGCAGTG1493100.083.712952
CAGTGGT1769150.070.528724
GTGGTAT1820450.068.203496
GGTATCA1834200.067.803448
TGGTATC1845250.067.231557
GTATCAA1887450.065.776079
GCAGTGG2099100.059.7910733
AGTGGTA2362600.052.7469375
CAACGCA1763000.039.00154512-13
ATCAACG1773400.038.75562310-11
AGAGTAC1860950.036.08096718-19
TACATGG1827000.035.5697722-23
AGTACAT1876200.035.39840720-21
TCAACGC1756350.035.23241412-13
GCAGAGT1960900.034.48666416-17
CGCAGAG1814300.034.2107116-17
GAGTACA1820800.033.2343920-21
GTACATG1822500.032.694522-23
TATCAAC1920650.032.4147810-11
Produced by FastQC (version 0.11.5)