FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36114409
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG392350.1086408474800183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1701800.094.62251
AGCAGTG1839200.087.124782
CAGTGGT2272250.070.386154
GTGGTAT2293450.069.4051746
GGTATCA2319700.068.681978
TGGTATC2357050.067.4331367
GTATCAA2368200.067.168959
GCAGTGG2495150.064.439313
AGTGGTA2569100.062.109565
CAACGCA2240400.039.31814612-13
ATCAACG2253550.039.03167310-11
AGAGTAC2359300.036.50723318-19
GCAGAGT2379550.036.30365816-17
AGTACAT2356050.036.14805220-21
TACATGG2300800.036.08339322-23
TCAACGC2234100.035.5587712-13
CGCAGAG2304350.034.5732216-17
GAGTACA2290450.033.9361820-21
GTACATG2278100.033.5416822-23
TATCAAC2395750.033.26211510-11
Produced by FastQC (version 0.11.5)