FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33451490
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG410860.12282263062123691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1503050.092.754541
AGCAGTG1641050.084.666032
CAGTGGT2019200.068.60234
GTGGTAT2031500.067.83466
GGTATCA2049650.067.362748
TGGTATC2071650.066.433027
GTATCAA2105050.065.438839
GCAGTGG2270000.061.471923
AGTGGTA2295100.060.210555
CAACGCA1965950.038.75602712-13
ATCAACG1984950.038.42215710-11
AGAGTAC2051350.036.13466318-19
TACATGG2000500.035.6869822-23
AGTACAT2074600.035.42783420-21
GCAGAGT2110050.035.38228616-17
TCAACGC1960400.034.9953912-13
CGCAGAG2035900.033.807616-17
GAGTACA2017700.033.10658620-21
GTACATG2012900.032.6799722-23
TATCAAC2126000.032.40399610-11
Produced by FastQC (version 0.11.5)