FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34841139
Sequences flagged as poor quality0
Sequence length126
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGA184700.0109.338151
ATAGACC191050.0108.783411
ACCGTAG142850.0107.686925
TGACGGC148900.0107.6866461
AGAGCGA144600.0107.277061
CATGGCG168050.0107.168071
GAGACGA162900.0106.9920359
CTAAGAC181300.0106.918511
AACCGCG215800.0106.79461
ATGCGAT169300.0106.447731
CGGACTG113350.0106.029131
ACTGATG147000.0106.0156561
ATCGCCA203850.0104.865237
GACCTGG195450.0104.4301451
ATCGAGT189800.0104.091281
ACCGAGA114200.0103.5974664
ATAACGC149350.0103.423011
AACTGCG129650.0103.380139
TCCGCTG206650.0103.241138
GGATGAC118300.0103.052052
Produced by FastQC (version 0.11.5)