FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34841139
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG586890.16844742073443697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC462660.1327912959447164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1631400.095.244891
AGCAGTG1815650.085.334072
CAGTGGT2181700.070.895624
GTGGTAT2253150.068.391566
GGTATCA2266450.068.003468
TGGTATC2274750.067.7817157
GTATCAA2337350.065.714769
GCAGTGG2501950.062.1300163
AGTGGTA2670600.057.7976655
CAACGCA2190700.039.16082412-13
ATCAACG2203050.038.8603810-11
AGAGTAC2324400.036.3984518-19
TACATGG2295450.036.07742722-23
AGTACAT2334700.035.95575320-21
GCAGAGT2394850.035.54141216-17
TCAACGC2190350.035.16887312-13
CGCAGAG2264500.034.18095416-17
GAGTACA2275550.033.3635120-21
GTACATG2297900.032.8062522-23
TATCAAC2361100.032.6577110-11
Produced by FastQC (version 0.11.5)