FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35815774
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG409290.11427646377263828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1720150.095.675231
AGCAGTG1897600.086.4539262
CAGTGGT2289100.071.5933764
GTGGTAT2361550.069.1058966
GGTATCA2366600.068.895048
TGGTATC2383350.068.365537
GTATCAA2442400.066.685639
GCAGTGG2579600.063.878273
AGTGGTA2670300.061.1839945
CAACGCA2293050.039.3725912-13
ATCAACG2313400.038.98150610-11
AGAGTAC2416800.036.66617618-19
TACATGG2374050.036.2506422-23
AGTACAT2435400.036.0633520-21
GCAGAGT2495050.035.70097416-17
TCAACGC2293250.035.49006712-13
CGCAGAG2366550.034.5156416-17
GAGTACA2377000.033.570520-21
GTACATG2374300.033.22551722-23
TATCAAC2459650.033.1699210-11
Produced by FastQC (version 0.11.5)