FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36367145
Sequences flagged as poor quality0
Sequence length126
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG126800.0106.663131
GACTACA203750.0106.125181
AACGATG140250.0105.917169
GCATGCG161300.0105.286081
CATGGCG182900.0104.930391
GCTGCGA150100.0104.7835161
GAGCGAC82150.0103.923142
CAGCCGA130550.0103.507311
TGAAGCG90150.0103.153065
GCGCGAT162100.0102.8777851
ACGCCGA111400.0102.547711
AGAGCGA125400.0102.109391
CGTGAGC99350.0101.7528461
CGCTGGA146350.0101.725851
CGAGTCG126400.0101.716455
GACGTGC167100.0101.7034761
CCGGTAG115800.0101.243241
ACTGAAC169850.0100.905061
CGAGAAG142850.0100.772341
CTCGGGA159750.0100.595811
Produced by FastQC (version 0.11.5)