FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36367145
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG539300.1482931915606793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC433470.11919274938959327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1990450.098.292991
AGCAGTG2229150.087.5335852
CAGTGGT2758600.070.60794
GTGGTAT2948400.065.826556
TGGTATC2963450.065.401137
GGTATCA2976750.065.155298
GCAGTGG3073150.063.7433053
GTATCAA3059600.063.2477879
AGTGGTA3171800.061.2026865
CAACGCA2907700.037.41423412-13
ATCAACG2923200.037.1928810-11
AGAGTAC3013350.035.44925318-19
TACATGG2968050.034.97693622-23
AGTACAT3034800.034.8820920-21
GCAGAGT3095500.034.65561716-17
TCAACGC2905300.033.32266612-13
CGCAGAG2982950.032.5162516-17
CATGGGG1657450.031.80962424-25
GAGTACA2985850.031.79139120-21
TATCAAC3070000.031.59295310-11
Produced by FastQC (version 0.11.5)