FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38225310
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG467490.1222985503583882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC410780.10746283025566046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3479000.0107.449531
AGCAGTG3822850.097.384052
CAGTGGT4656900.079.802854
GCAGTGG4819050.077.3804243
GTGGTAT4813850.077.2066356
GGTATCA4888650.076.516928
AGTGGTA4845500.076.480365
TGGTATC4871850.076.193887
GTATCAA5010650.074.862249
CAACGCA4793750.042.8802612-13
ATCAACG4909200.042.0084310-11
AGAGTAC4803350.041.43562318-19
TACATGG4603350.041.40387322-23
GCAGAGT4862400.041.1242916-17
AGTACAT4851700.040.58383620-21
ACGCAGA5105550.040.1747414-15
TCAACGC4802150.038.92670412-13
CATGGGG2465400.038.06200424-25
CGCAGAG4885300.037.9161116-17
GAGTACA4791550.037.66332620-21
Produced by FastQC (version 0.11.5)