FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42752403
Sequences flagged as poor quality0
Sequence length126
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA273950.0107.455511
CGATGGC278150.0106.653031
AGAGCGA154100.0104.6291961
GACTAAC144300.0104.247181
GCATGCG200350.0103.9056851
CGAGCAC157050.0103.657631
ATCCGAA227200.0102.643681
CGCTTCA235050.0102.561181
GACGTGC190500.0102.314421
CTCGGGA199900.0102.157691
TCCACGA192900.0101.917288
GAAGCTA127450.0101.534576
TGGACGC125000.0101.086141
GATGCGA87100.0101.045179
AGCGATA151600.0101.031148
CGGACTG120050.0100.854031
GATCTAG181600.0100.750831
GTACGTA232550.0100.256471
TAGATGC138700.099.7871867
CGACCAA170600.099.7319642
Produced by FastQC (version 0.11.5)