FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42752403
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3268550.0104.750531
AGCAGTG3599650.094.676072
CAGTGGT4587150.074.168984
GCAGTGG4823550.070.7915653
AGTGGTA4822250.070.3480155
GTGGTAT4970300.068.435756
TGGTATC5015900.067.716697
GGTATCA5078800.067.292498
GTATCAA5237150.065.430849
CAACGCA5021100.037.5902812-13
ATCAACG5101000.037.09340310-11
AGAGTAC5030300.036.19094518-19
TACATGG4829150.036.12254722-23
AGTACAT5051400.035.59525720-21
ACGCAGA5335750.035.31569714-15
GCAGAGT5213800.035.15545716-17
TCAACGC5023800.033.9505412-13
CATGGGG2881750.033.34312424-25
CGCAGAG5123850.033.0440716-17
GTACATG4914650.032.73727422-23
Produced by FastQC (version 0.11.5)