FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35455956
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA328550.0110.83681
CGCAACG215400.0110.103732
AGCTTGA264650.0109.7654651
GCGCAAC282250.0109.3920751
AGACTAG198750.0109.3647168
CATGGCG195200.0108.836261
GTGATGC210950.0108.215928
AGTGATG212050.0107.909187
CTCGAAA205300.0107.6668551
CTGCGGA131400.0107.634681
AGCCGGA196500.0107.4734042
TGCTACC231000.0107.106431
GATGCTA164600.0106.730541
CGATCGC153350.0106.6493155
AGCGATA135800.0106.648198
TGACTAG167300.0106.0495151
AGTAAGA251500.0105.725019
TGATGCC216150.0105.5570149
GCAACGT226000.0105.2581253
GACTAAC125400.0105.166671
Produced by FastQC (version 0.11.5)