FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32964575
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT900100.080.1111151
AGCAGTG990850.072.6195142
GGTATCA1085000.066.605768
GTGGTAT1097750.065.558786
TGGTATC1125400.063.964057
CAGTGGT1170000.061.7796864
GTATCAA1205450.060.0596589
AGTGGTA1217350.059.031945
GCAGTGG1549700.046.8497923
CAACGCA1015050.039.5017612-13
ATCAACG1046500.038.44938310-11
TCAACGC1018150.035.5393912-13
TACATGG1123150.034.6000122-23
GCAGAGT1163900.034.04327816-17
CGCAGAG1071500.033.6500816-17
AGTACAT1207500.032.7133620-21
ACGCAGA1257500.031.99796114-15
GAGTACA1115450.031.90312820-21
AGAGTAC1286950.030.74864818-19
GTACATG1154350.030.66818222-23
Produced by FastQC (version 0.11.5)