FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35983955
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA222850.0110.0565951
CTAAGAC193800.0110.0292051
CTTACCG285350.0109.281141
GAACGAG153650.0108.941793
TGACGGC125350.0108.375711
TGGACGC158750.0107.942271
GATCGTA200600.0107.826951
TGAACGA155200.0107.4671862
CGGAGCT200150.0106.598431
CGGACAC175800.0106.4962541
GTCGAAC135550.0106.314558
AGCGTCG75700.0106.2772751
TTGAACG248700.0106.154891
ATGCGAT182950.0105.881081
ACATACG216000.0105.563491
ACGATAC146500.0105.361571
GATGAAA285650.0105.1068041
TCGGGAC192900.0105.091741
ACGAGAG103450.0104.970519
TCGACGG123150.0104.79138
Produced by FastQC (version 0.11.5)