FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35983955
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT856300.066.309661
AGCAGTG912950.062.1122672
GTGGTAT921850.061.6152236
GGTATCA940200.060.534098
GTATCAA1009100.056.542819
TGGTATC1037100.054.872237
CAGTGGT1138550.050.2287944
AGTGGTA1166400.048.6282735
GCAGTGG1462150.039.3403933
CAACGCA813800.038.9118212-13
ATCAACG870100.036.61822510-11
TCAACGC826650.034.32238812-13
TACATGG951550.032.3807622-23
GCAGAGT994400.031.56203316-17
CGCAGAG911850.031.00110416-17
AGTACAT1013650.030.77713420-21
GAGTACA918450.030.31544120-21
AGAGTAC1069500.029.27567118-19
GTACATG964100.028.73842822-23
ACATGGG984650.027.62102724-25
Produced by FastQC (version 0.11.5)