FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36762349
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC234250.0108.6459053
GAGCAGA201950.0108.084491
GTGCAGC195750.0108.008841
GAATAGC159150.0107.702532
ACACTGA309100.0107.217831
TACGGGC232500.0106.958241
TAGCACG194900.0106.907649
TCGTAGA146200.0106.478471
CAGCCGA132800.0106.228681
CAGCGAC119400.0105.7718661
ATAACGC144700.0105.506181
TAGCGAC118950.0105.413847
CGGACTG104650.0105.293571
ACGAAGT188150.0105.2600563
CACTGAT241350.0105.101762
CTGCGGA110100.0104.9928051
TGCGAGA84850.0104.860434
GGCTCGA135100.0104.375741
GTACTAG167100.0104.301843
GTGCGAG113000.0103.957673
Produced by FastQC (version 0.11.5)