FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36374837
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1296200.090.239171
AGCAGTG1441000.081.1149752
GTGGTAT1713600.068.369426
GGTATCA1729250.067.927618
CAGTGGT1750600.067.054394
TGGTATC1763900.066.385767
GTATCAA1816700.064.766089
AGTGGTA1847150.063.293945
GCAGTGG2121100.055.4291533
CAACGCA1638400.039.79238512-13
ATCAACG1668800.039.21315410-11
TACATGG1761000.036.15561722-23
TCAACGC1646200.035.63488812-13
GCAGAGT1818050.035.574416-17
AGTACAT1815850.035.47659320-21
CGCAGAG1710650.034.15317516-17
AGAGTAC1913600.033.76674718-19
ACGCAGA1945800.033.59088514-15
GAGTACA1735200.033.3788920-21
GTACATG1770350.032.60748722-23
Produced by FastQC (version 0.11.5)