FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35587727
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGA162650.0110.4011152
CACGCGA194500.0110.366648
GCGTCTG238900.0109.970425
ATGCGAT231250.0109.82121
CAGGACG155150.0109.201868
ACAGCCG209600.0109.097091
ACCAGTA341450.0108.900291
ATCCGAA183600.0108.4137651
CTCGAAA241100.0108.324851
TGGCGAG166600.0108.1076663
GCGATCA230650.0107.688093
ATCCGCG174400.0107.399829
TACTCGA132450.0106.8998645
CCTCTGA406900.0106.843111
GATTCAG238700.0106.670461
TAGACCA222900.0106.657561
AGCGTAT227550.0105.9037861
AGTGACG136200.0105.76267
ACGAGGA130650.0105.675991
CGAGGAC121500.0105.67162
Produced by FastQC (version 0.11.5)