FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35587727
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1288400.084.1951141
AGCAGTG1388700.077.9444662
GTGGTAT1606900.067.495876
GGTATCA1664750.065.319878
TGGTATC1728700.062.757637
GTATCAA1737750.062.644339
CAGTGGT1746800.062.219484
AGTGGTA1797400.060.1583375
GCAGTGG2035600.053.5368463
CAACGCA1529950.039.4938612-13
ATCAACG1585300.038.27595510-11
TACATGG1635450.035.96404622-23
TCAACGC1540150.035.3094612-13
GCAGAGT1696400.035.24276716-17
AGTACAT1701650.034.92683820-21
CGCAGAG1621550.033.38221716-17
AGAGTAC1808900.033.0028418-19
GAGTACA1633600.032.7290820-21
GTACATG1662600.032.0781822-23
ACGCAGA1918250.031.62615614-15
Produced by FastQC (version 0.11.5)