FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37383192
Sequences flagged as poor quality0
Sequence length126
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG359450.0114.47792
TGACTCA448250.0113.7739
CGTCTAG457000.0113.7636267
GACGCGT384000.0113.620631
GTGACTC450000.0113.386328
GTCTAGC461050.0113.349618
CAGGCGT397700.0113.022043
TGGAGAC395750.0112.648175
GACTGCG265900.0112.550561
CGGGATC313200.0112.52622
GCGTCGC397200.0112.251466
TGTGACT449800.0112.1564567
GGTAGTC440050.0112.054444
TCAGGAG277100.0112.017243
CGTGTGA366100.0111.905145
TCGTCTA459600.0111.880596
TCTACAA559950.0111.774082
CGGACTG223350.0111.740931
ATGTCGT516850.0111.5094763
ATGACGG209050.0111.4729849
Produced by FastQC (version 0.11.5)