FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37383192
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1299200.087.109051
AGCAGTG1377700.081.984522
GTGGTAT1435700.079.232896
GGTATCA1420050.079.211158
GTATCAA1499650.075.07079
TGGTATC1505500.074.767047
CAGTGGT1525350.074.141244
GCAGTGG1610200.070.392443
AGTGGTA1685550.067.332495
CAACGCA1333800.045.2212812-13
ATCAACG1360300.044.5784610-11
TCAACGC1335400.041.7035912-13
TACATGG1430450.039.92877622-23
CGCAGAG1392650.039.8942216-17
GCAGAGT1503300.039.71353516-17
GAGTACA1387100.039.0780920-21
AGTACAT1514350.038.64303220-21
AGAGTAC1564900.037.68875518-19
GTACATG1447400.037.05898322-23
ACGCAGA1677800.036.1718214-15
Produced by FastQC (version 0.11.5)