FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37710272
Sequences flagged as poor quality0
Sequence length126
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC201300.0110.832181
CGGACTG170400.0110.499931
CGGACAC231800.0108.315721
TGACGGC121750.0107.4249951
GATCGCG124500.0106.54671
GACTGCG163250.0106.294791
CTAATGG191200.0105.636551
GATGCTA138550.0104.709771
AGATGCC150000.0104.3973548
GCGAGAC62400.0104.229235
CGCTATC218650.0103.767031
CATCGAA159000.0103.548961
AGATAGC144750.0103.541418
ACTAGAG114550.0103.401913
AGGCGAC74050.0103.3059548
ATGCGAT132950.0103.0254061
AGCTTGA134050.0102.985971
GCTAGAA117050.0102.826335
TACGGAT168300.0102.499011
AGAGCGA105350.0102.440621
Produced by FastQC (version 0.11.5)