FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38775463
Sequences flagged as poor quality0
Sequence length126
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG201850.0109.667461
GTAACTG191200.0106.95471
TGACGGC132650.0106.832741
CACGAGA145500.0106.101528
ACGAGAG145550.0106.064119
TACGGAT215350.0105.579781
ACTGATG119650.0105.2968441
AGCGAGA115400.0104.8684548
CAATGGA192600.0104.8270958
GTACGCG102800.0104.5889369
TGACAAC185150.0104.387331
GGCGACT153300.0104.1489941
TGAGCCG120450.0104.049331
TGAGCAT173500.0103.993251
CCTTACG200750.0103.989541
TGACTAG140050.0103.7165151
ATGCGAG75700.0103.443
CTCACGA137250.0102.99346
TACGGTA199400.0102.646671
TGAGCTA113550.0102.508796
Produced by FastQC (version 0.11.5)