FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38775463
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1313050.083.419631
AGCAGTG1372700.079.545542
GTGGTAT1554000.071.664146
GGTATCA1512750.071.621328
GTATCAA1561400.069.624219
TGGTATC1563950.069.265117
CAGTGGT1618250.067.407154
GCAGTGG1784600.061.4717183
AGTGGTA1834300.060.543365
CAACGCA1391900.041.4068912-13
ATCAACG1432000.040.88443810-11
AGAGTAC1424200.038.3537718-19
TCAACGC1389650.037.75498212-13
CGCAGAG1455300.035.8498316-17
GCAGAGT1574000.035.55526416-17
TACATGG1453700.035.48501222-23
GAGTACA1418150.034.82541320-21
TATCAAC1533950.034.73954410-11
AGTACAT1557600.034.59651620-21
GTACATG1427950.033.5975322-23
Produced by FastQC (version 0.11.5)