FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36547894
Sequences flagged as poor quality0
Sequence length126
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACC141050.0108.129871
TGAGCCG155400.0107.994151
ACTGAAC146300.0107.983061
GTGAACG120200.0107.218519
CGTGAGC100150.0105.7810441
GATCGCG204800.0105.4791
CGTTGAC159850.0104.570329
GATGCTA140900.0103.899841
TGACAAC196100.0103.730881
TACGGAT196600.0103.589191
TGACGGC90600.0103.48251
GTCGACT139000.0103.466078
CAGCGAC105700.0103.463571
CTCGAAA149400.0103.452431
GTAACTG151650.0103.104921
TCGAGCG50250.0102.92655
CGAGCGA50900.0102.554026
AGTACGA130200.0102.3026058
GGCGACT86550.0102.222021
TGCGAGA69950.0102.162884
Produced by FastQC (version 0.11.5)