FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34808577
Sequences flagged as poor quality0
Sequence length126
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG510350.0115.54219
ACTAGAG407800.0115.5388643
GAGATGA463100.0114.750639
ATGCGAT416400.0114.5103151
GATAGGC311100.0114.467135
AGTACGC521100.0114.0695958
CCGATAG321650.0113.967643
TTCGTAG408550.0113.949037
GCTTGAG412150.0113.892952
CGATAGG313550.0113.843894
AGAGATG467000.0113.81958
ACCAGCG355500.0113.723825
CGATTCG409450.0113.631174
GCCGATA318350.0113.571662
TACCAGC356950.0113.281924
GCTGTCG347950.0113.208215
GACGTCG244550.0113.0681842
TCGCGTG382150.0113.058229
CGGGATA324600.0113.012032
ATTCGTA409300.0112.9642646
Produced by FastQC (version 0.11.5)