FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44568882
Sequences flagged as poor quality0
Sequence length126
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC227850.0109.238291
CTGCGGA155950.0108.185871
GATGCAA183150.0107.8611154
AGATGCA202500.0107.300943
GATGCTA195100.0106.949011
CTAAGAC180650.0105.462831
ACGGAGA193850.0105.0979461
TCGTGAC148050.0105.0729148
AGCTAGA122300.0104.9705354
CTGATAG175900.0104.9303051
ATAACGC156450.0104.845621
ACTGAAC158800.0104.844811
CCGAACG131700.0104.41219
TCTGCGA137000.0104.220488
GCGAAGA108850.0103.666984
GAGCGAA135600.0103.611342
CGATGGC173350.0103.35551
GCATAAG153050.0103.204263
AGCGTCG173950.0102.239371
AGCGAAG194650.0102.197483
Produced by FastQC (version 0.11.5)