FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37352848
Sequences flagged as poor quality0
Sequence length126
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACGC272950.0114.869881
CGTCTAC532350.0114.352391
CGGTACA563450.0114.348273
GCGGTAC462250.0114.1664052
TGACGGC190750.0114.146391
CGCGGTA479150.0113.936631
ATGAGTC388250.0113.568154
AGTCTAG398250.0113.1001367
GACTAAC315700.0112.937471
CAGCTGC569850.0112.393878
CCTTACG422900.0112.289171
AGTGCAC248050.0111.761287
GATGAGA347000.0111.6565259
GGATGAG398150.0111.557912
GTCGTAA241650.0111.379911
GAGTGCA300150.0110.826576
GATGAGT401550.0110.5834353
TGAGCAT305400.0110.09881
GGGATGA420550.0110.060151
GAGTCTA403250.0109.983376
Produced by FastQC (version 0.11.5)