FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37352848
Sequences flagged as poor quality0
Sequence length126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG615440.16476387556847072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC513730.13753435882586518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA480880.12873984869908714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2258150.098.400811
AGCAGTG2417100.091.7351762
CAGTGGT2980100.074.3064964
GCAGTGG3086900.072.005343
GTGGTAT3087900.071.8061146
GGTATCA3100200.071.4583748
TGGTATC3128850.070.6479957
GTATCAA3187200.069.6236659
AGTGGTA3375800.065.565425
ATCAACG3036500.040.18586310-11
CAACGCA3027750.040.14375712-13
AGAGTAC3056000.038.35088718-19
GCAGAGT3128650.037.71962716-17
TACATGG2984500.037.7188922-23
AGTACAT3110100.037.2559920-21
TCAACGC3009900.036.69100612-13
CGCAGAG3051450.035.87856316-17
GAGTACA3008000.035.2983820-21
GTACATG3029500.034.44119322-23
CAGAGTA3135250.034.17842518-19
Produced by FastQC (version 0.11.5)