FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41943764
Sequences flagged as poor quality0
Sequence length126
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTC617250.0115.693793
GGACTCA518050.0114.757254
TAGGACT624750.0114.34332
ACGAGGA380100.0114.0929641
CTGATCC562700.0113.9703451
GAGTTGA563250.0113.742587
CGAGGAG370150.0113.432222
TTAGGAC643550.0113.381461
GATGCTA318150.0113.181851
AGTTGAG620100.0112.911998
TGGTACC523100.0112.559916
GGTACCA523600.0112.490827
GTTGAGA576400.0112.308589
TACCATC695750.0112.220729
GTACCAT699400.0111.646648
ACAGCCG308350.0111.616961
AGAGTTG574350.0111.435936
TCGCGGA162850.0111.179638
ATCGTAA277450.0110.509432
TGATCCC492050.0110.506762
Produced by FastQC (version 0.11.5)