FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41943764
Sequences flagged as poor quality0
Sequence length126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1136800.2710295623444763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC941300.22441953468935213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3798500.0104.856571
AGCAGTG4092050.097.1662452
CAGTGGT5110400.077.637254
GCAGTGG5189900.076.699943
GTGGTAT5440450.073.001176
TGGTATC5474650.072.308877
GGTATCA5498200.072.236848
AGTGGTA5560100.071.270885
GTATCAA5608900.070.90289
ATCAACG5433300.040.216910-11
CAACGCA5411100.040.19231412-13
AGAGTAC5396750.038.9161618-19
GCAGAGT5477700.038.56769616-17
TACATGG5217650.038.51719722-23
AGTACAT5423700.038.26428620-21
TCAACGC5393700.036.70314812-13
ACGCAGA5954500.036.4596214-15
CGCAGAG5432700.036.09479516-17
CATGGGA2051150.036.03661324-25
GAGTACA5340800.035.6870220-21
Produced by FastQC (version 0.11.5)