FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44229943
Sequences flagged as poor quality0
Sequence length126
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA270050.0108.94751
AGATAGC162750.0108.151998
TGACGGC115450.0105.047341
CGGACGT183400.0104.709481
TGAGCCG155800.0104.59841
CCTTACG235450.0104.471521
CAGTAAC257800.0104.2218251
CGGACTG124050.0104.108071
AGCTTGA178250.0103.85941
CGATTGA185700.0103.833091
CTGCGGA109350.0103.326731
CACGAGT166700.0103.282793
GGCGACT145350.0103.233371
ATAACGC148450.0103.222161
TGACTAG156200.0102.7538451
AGGCGAC76300.0102.21498
ACACGAG136000.0101.983772
CGCTATC217900.0101.831551
GTAACCA193750.0101.4959953
GCGCGAT114750.0101.133991
Produced by FastQC (version 0.11.5)