FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16977631
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG81900.019.3394475
TACACTG87400.016.5091615
CTATACT58950.016.105184
TATACAG75750.015.3254195
GTATTAG54750.015.1146921
TCTATAC52750.014.9704043
CCTATAC38400.014.6891513
GTGTAGG62700.014.6230461
GTATAGG34150.014.594391
TATACGG10200.014.2843225
TAGACTG69950.014.1772765
GTGTACG23250.014.1561731
GTCCTAG55400.014.0886411
GTATATA117000.013.9046821
TACACAG108300.013.8439595
GTGTAGA71700.013.6400191
GTCTTAG50250.013.3804511
GTATAGA53300.013.32051
TACACCA85750.013.2641465
GTGTATA81600.013.2528261
Produced by FastQC (version 0.11.5)