FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11715338
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG50500.016.6588485
CTATACT35450.015.90924
GTATAGA35400.015.00616551
GTGTAGG45000.014.938881
GTATAGG25800.014.75911
GTATACG6800.014.5179381
GTATTAG37300.014.4938411
CCTATAC24650.014.4911293
GTGTATA48550.014.04021
CTAGACT31450.013.8977854
CTACACT45150.013.8444984
TACACTG58500.013.5773235
GTATAAG32300.013.5355211
GTCCTAG34500.013.4899591
TATACAG47300.013.4139275
CGTACAC14450.013.33587553
TAGACTG41450.013.2661415
CTAGGAC33700.013.2494933
TACACAG71200.012.9377695
CCTACAC40000.012.8077273
Produced by FastQC (version 0.11.5)