FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11715338
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG48500.017.1512325
GTCGCCG48800.017.0433994
TGGTCGC67900.015.98618294
TCGCCGT38800.015.86507594
CGCCGTA23000.015.52702794
TACACTG58000.015.2332885
GTATTAG35100.015.01483251
GTGTAGG45050.014.6232171
GTGTTAG42900.014.5882761
CTACACT45900.014.5388544
GTACTAG17900.014.4583681
TACACAG72650.014.1668175
CCTACAC41600.014.12945653
GTATAGG25000.014.1166251
GTCTAGG32150.014.0507631
TAGACTG43050.013.97335155
GTGGTCG85200.013.95351194
GTGTATA49850.013.875921
GGTCGCC63900.013.82482294
GTATAAG31200.013.7244971
Produced by FastQC (version 0.11.5)